FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48525045
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT633210.1304913782151052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC179850.021.0549934
CAGACCG185200.020.6405743
ACGCTAC165950.019.11514724
CGCTACC170950.018.38497725
AACGCTA206100.018.31680723
GAACGAT206550.018.3115245
GACCGCG167050.018.19665
TCACGCT218350.017.9711028
GCCGGCT200800.017.7837581
ACCGCGT177800.017.0964136
CCGGCTT239300.017.0742842
TTCACGC232050.016.9973727
AAAACGC227000.016.68969721
GAAAACG232000.016.30091320
CCGCGTT188250.016.147377
CGCGTTC190650.015.9440988
AAACGCT238950.015.88328322
GTCCGTT36950.015.72133351
TCAGACC250700.015.61582
ACGCTCA234250.015.578073510
Produced by FastQC (version 0.11.5)