FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48525045
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG583770.12030282506693193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG193800.015.37059945
GGGAGGT270750.015.0074133
GGCATAG208350.014.24320935
ATAGGGG208850.014.23065538
GTCACTC281400.013.9757098
GAGCTTA226800.013.19365544
GGAGGTC306750.013.18014054
GAGGTCA317300.012.7702735
ACTCTCC307600.012.7487511
TCACTCT318550.012.4376489
TGGCATA243050.012.35782934
CATAGGG251000.012.02916137
TGGGAGG270200.011.8238682
CACTCTC331550.011.7192510
GCATAGG258600.011.68433636
GGTCACT357050.011.01460557
GCTCTGT374400.010.55826322
GTTGTCG21900.010.27335726
TCAGACG73950.010.0399682
TCTCCCC397900.09.9855813
Produced by FastQC (version 0.11.5)