FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49112987
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1342390.2733268900952817No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC514710.10480120054599815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG24500.023.8694576
GAACGAT283700.023.31453345
ACCGCGT312900.021.3134866
GACCGCG315400.021.153555
AGACCGC318300.020.9608214
CCGCGTT322100.020.6977337
CGCGTTC327900.020.3727828
CAGACCG332900.020.2240033
AGAACGA347150.019.13111944
GTTCCTA108500.019.07187715
TCAGACC367150.018.484882
GCGTTCT374700.017.906269
CCGTACG62900.017.773774
CTCAGAC420650.017.345351
CGTACGC64850.017.1699495
AAGAACG387200.017.14437543
CGTTACG23700.016.89369621
GCACTAC83700.016.87672225
TCGACGA36700.016.7313847
AATACGG397550.016.59331332
Produced by FastQC (version 0.11.5)