FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47007513
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2035570.43303077956921476No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC547600.1164920169250392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT301600.032.41340645
AGACCGC356850.027.4945144
CAGACCG361700.027.231613
AGAACGA363900.026.9631344
CGCGTTC377250.025.872298
CCGCGTT377650.025.8512297
GACCGCG379650.025.8136865
ACCGCGT383200.025.4826936
AATACGG392650.024.94322232
AAGAACG395150.024.87643643
ATACGGA395550.024.78281233
TCAGACC401650.024.7385862
TACGGAG399650.024.48345234
CAATACG416900.023.562531
CCCAATA428350.022.95367229
ACGGAGA430100.022.80762535
CTCAGAC456200.022.7708421
GCGTTCT431950.022.6636689
CGGAGAG436900.022.38564936
CCAATAC462750.021.42237330
Produced by FastQC (version 0.11.5)