FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45382388
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1107770.24409689503337725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT221900.023.24625645
AGACCGC239750.021.1940164
CAGACCG258700.019.9198913
AGAACGA264900.019.49827644
GACCGCG263700.019.2435135
ACCGCGT266900.019.0465186
CCGCGTT277700.018.3219877
CGCGTTC277950.018.305518
TCAGACC296050.017.7124142
CTCAGAC329700.017.1826341
AAGAACG303500.017.06290243
AATACGG303350.016.96094132
ATACGGA304250.016.88879433
TACGGAG303400.016.8693934
TTCGACG14550.016.8582086
GCGTTCT305050.016.7973189
CAATACG317050.016.29190831
CGTTACG15400.016.22419521
ACGGAGA325000.015.78283535
CCGTACG70400.015.4710814
Produced by FastQC (version 0.11.5)