FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45382388
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG137500.014.569715545
ATAGGGG148800.013.46066538
GGCATAG147950.013.38373435
GGGAGGT235050.012.5668093
GAGCTTA160150.012.46695644
TGGCATA166700.012.08065134
CATAGGG170650.011.82893237
GCATAGG180350.011.10449436
GGAGGTC254500.011.085624
TATCCTA94450.010.958795
ACTCTCC258950.010.94053511
GTTGTCG18050.010.71759426
GGTCACT260450.010.5060147
ATCCTAG108700.010.47489556
GTCACTC268150.010.4560818
TAGGCTA98800.010.044085510
TCAGACG79350.010.0362872
TGTATCC113350.09.90374853
CCTAGGC105900.09.62566958
CACTCTC292800.09.55275910
Produced by FastQC (version 0.11.5)