FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44063435
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3385710.7683717803661925No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG706120.16025078389825942No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA658600.14946633189173744No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG627940.14250818167035775No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC456060.10350078245148159No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG444270.1008250945483483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT539700.030.17832845
AGACCGC614450.026.9066944
CAGACCG617000.026.8037283
GACCGCG624450.026.4361745
ACCGCGT631200.026.181576
AGAACGA628850.025.95993444
CCGCGTT648100.025.5161537
CGCGTTC650850.025.4048548
TCAGACC669950.024.9275912
CTCAGAC713050.024.331311
GCGTTCT680050.024.3173319
AAGAACG690050.023.622643
AATACGG708500.022.89861332
ATACGGA709350.022.8327733
CAATACG721950.022.57745731
CGTTCTC743400.022.44786810
TACGGAG723400.022.31432534
ACGGAGA734950.021.93288635
TTCGACG23400.021.7301866
CCTCTCA767800.021.69108219
Produced by FastQC (version 0.11.5)