FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44063435
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG632860.14362475372153805No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG609440.13830968920148873No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA607980.13797834871475634No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG546880.12411197629054567No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT442480.10041886203379287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT302450.014.80892853
GGTCACT316600.013.8057357
AGCTTAG225900.013.6774745
GTCACTC322050.013.5728878
GTTGTCG23750.013.35846726
GGAGGTC339050.013.3211394
ATAGGGG233850.013.20141138
GGCATAG233250.013.19607735
ACTCTCC349200.012.44773111
GAGCTTA250250.012.32822344
TGGCATA259450.011.97614334
TCACTCT372950.011.9499469
CACTCTC368250.011.79126710
AGGTCAC399200.011.4279186
TGGGAGG292000.011.4271452
GAGGTCA414700.011.141255
CTGGGAG364750.010.3178831
CATAGGG306300.010.18894337
CTCCCCA323850.010.039721514
GCTCTGT439000.09.98478922
Produced by FastQC (version 0.11.5)