FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45859346
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG526830.11487952750132983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG177050.015.45860845
ATAGGGG190100.014.59997638
GGCATAG188100.014.44468935
GGGAGGT290200.014.3890133
GAGCTTA202500.013.5911144
GGAGGTC304350.013.3423414
GTCACTC299800.013.2346978
TGGCATA208350.013.20250434
CATAGGG227650.012.19183337
ACTCTCC330250.012.12676211
TCACTCT349150.011.7179119
GAGGTCA365850.011.5789155
GCATAGG243450.011.41004136
TGGGAGG266400.010.9043912
CACTCTC369500.010.87104710
TAGGGGA367000.010.79962739
GGTCACT386400.010.4025497
TCTCCCC392900.010.319097513
GCTCTGT396850.010.13195822
TGTCCAA403900.010.11197926
Produced by FastQC (version 0.11.5)