FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45926579
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG987960.21511726357846075No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT908450.1978048484734733No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG517080.11258839897480716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT260700.019.908621
CCGGCTT308350.019.0351072
AGACCGC239900.018.8303624
GACCGCG240600.018.6914235
ACCGCGT242500.018.6191926
TTCGACG20450.018.3716586
CAGACCG253200.018.018953
CCGCGTT256900.017.4792027
TTCACGC320650.017.4419527
CGCGTTC258950.017.410338
CGGCTTC354050.017.1372663
TCACGCT331550.016.8006768
GTCCGTT36050.016.2327061
CGTTACG18800.015.91831321
TCAGACC294150.015.8928532
ACGCTCA362400.015.64362910
GCGTTCT291000.015.5546489
CACGCTC365150.015.2978569
CTCAGAC342300.015.0181
GTTCCTA87650.014.86124415
Produced by FastQC (version 0.11.5)