FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48388037
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA535950.11076084776904672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG32600.016.01252726
GGGAGGT308000.012.7791133
AGCTTAG202750.012.01210545
TGTCGTT43800.011.91845628
ATATAGC55050.011.4407386
GTCGTTC48400.011.39067229
GGAGGTC340750.011.3144
GGTCACT334800.011.0721367
GTCACTC334200.011.065398
GAGGTCA356400.010.89304355
GGCATAG225400.010.80344435
ATAGGGG230150.010.76518738
GCTAAGT52900.010.6318814
ACTCTCC351600.010.47977711
GAGCTTA236650.010.338922544
TCGTTCC51050.010.31497230
TCACTCT372050.010.1152739
CGTTCCC54150.010.09898731
CACTCTC376950.09.74513110
TGGCATA255500.09.61858534
Produced by FastQC (version 0.11.5)