FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49632754
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCTC65250.09.96505339
CACTCGA105100.09.4682915
AGCTTAG94700.09.3363945
ATAGGGG99650.09.324656538
ACACTCG115000.09.3196824
GGCATAG97050.09.20363535
GTCACTC153050.08.7610978
GGTCACT151050.08.6975577
ACCCGGC84400.08.6694785
CATAGGG103750.08.60930637
GGCGTAG47750.08.38708635
GCATAGG108150.08.38402736
ACTCTCC163100.08.30565111
TAGCGTA4651.7191633E-78.22548937
GCGTAGG49150.08.19414536
CGTAGGG49200.08.1856637
TAGCTCT80300.08.04150740
GATAGCT84400.07.94390238
ACTCGAG143500.07.8182966
TGATAGC87850.07.811309337
Produced by FastQC (version 0.11.5)