FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57019403
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1235710.21671745668750692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC271350.022.342924
CAGACCG277800.022.0185813
GACCGCG301150.020.1170525
ACCGCGT322700.018.8613436
GAACGAT325950.018.25500145
CCGCGTT336250.018.0970767
CGCGTTC338800.017.965798
TCAGACC345350.017.9398542
CTCAGAC382900.017.3677791
GCGTTCT352800.017.3038679
TTCGACG23100.016.364466
CGTTACG21150.016.27418721
AGAACGA384800.015.65022344
GCCGGCT205700.015.6121191
CCCGGAT152850.015.1797771
CCGGCTT248900.015.0091242
AATACGG404850.014.99034832
CCCGTAT41350.014.9123881
ATACGGA410050.014.82247433
TACGGAG410850.014.71145534
Produced by FastQC (version 0.11.5)