FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49862867
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1165880.23381728130474327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC274650.026.020374
CAGACCG281150.025.4375153
GACCGCG292400.024.4071485
ACCGCGT298300.023.9318736
GAACGAT300400.023.19366545
CCGCGTT308200.023.1843157
CGCGTTC313300.022.7850258
TCAGACC338700.021.4478912
GCGTTCT340850.021.0286319
AGAACGA336950.020.637644
CTCAGAC381200.020.5640241
AATACGG366050.019.12995532
ATACGGG56900.018.50752633
AAGAACG376800.018.44348743
CAATACG383100.018.37792231
CGTTCTC395900.018.34899910
TACGGAG328750.018.12457534
CCGTACG87950.017.9603124
ATACGGA333250.017.95407133
CCCAATA398750.017.75533729
Produced by FastQC (version 0.11.5)