FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49862867
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG638740.12809933291641654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG205000.014.58344145
GGGAGGT333150.014.1366363
GGCATAG219950.013.63658535
ATAGGGG222700.013.50851838
GAGCTTA230950.013.0906144
GGAGGTC360000.012.9354354
TGGCATA237300.012.6228234
GGTCACT374100.012.0408067
GTCACTC373550.011.9504348
TGGGAGG272100.011.6979072
GTTGTCG17950.010.91397226
CATAGGG280900.010.7740437
TCACTCT433050.010.58375559
TCTCCCC435550.010.36814613
TCAGACG85250.010.2571972
GCATAGG299550.010.17846236
CACCCGG117800.010.1018621
CTGGGAG368500.010.0602951
ACCCGGC71350.010.05365855
CTCCCCA321250.09.86643314
Produced by FastQC (version 0.11.5)