FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45863671
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1973400.4302751953719535No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC526280.11474877359904313No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA472960.10312301429163835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT328300.028.75995645
AGAACGA382450.024.82343544
AGACCGC383250.024.7159124
GACCGCG382350.024.7093735
CAGACCG385800.024.5876123
ACCGCGT388850.024.3126036
CCGCGTT397850.023.7624597
CGCGTTC403300.023.4580758
TTCGGCG113400.022.3952544
TCAGACC434500.022.184242
AATACGG438350.021.69428832
TACGGAG440500.021.48735634
ATACGGA442600.021.43577433
GCGTTCT449200.021.1261849
TCGGCGC121300.021.12247345
AAGAACG449200.021.12067443
CTCAGAC485850.020.847221
ACGGAGA470050.020.13755235
CAATACG472900.020.13201731
CGGAGAG472400.020.01913336
Produced by FastQC (version 0.11.5)