FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45863671
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG488090.10642192161198784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT27850.018.4788411
TCGTGTA31000.017.1146514
GTCGTGT38200.013.7711813
ACCCGGC80700.012.6718285
GTCACTC220600.012.5592338
GGTCACT231250.012.2707527
GGCATAG159100.012.0053535
AGCTTAG161300.011.927443545
TAGCGTA7600.011.88049337
ATAGCTC76800.011.86616439
TGTAGGG46750.011.54150817
TAGCTCT79600.011.50569240
GGGAGGT265600.011.4605633
GTCGCCG62250.011.3332444
GTAGGGA48750.011.29760518
ATAGGGG176250.011.07183538
TCACTCT262750.010.9295139
AGTGTAG50200.010.77124628
TCAGACG93050.010.7030482
GGTCGCC50700.010.67925943
Produced by FastQC (version 0.11.5)