FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51989679
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT790740.15209557266164309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT152000.024.6558745
TTCGACG35250.024.5775876
CGGTATA56900.019.41730320
AGACCGC202200.018.5204894
CAGACCG207450.018.1168753
AGAACGA212600.017.71285644
CGACGAG50600.017.563728
AATACGG221650.016.86167332
ATACGGA222050.016.82142433
TACGGAG225500.016.62403534
CGCAAAC71250.015.94268925
CCGCGTT236950.015.8597897
CGCGTTC240800.015.60378558
CAATACG245650.015.37000431
GACCGCG243550.015.3298435
TCGACGA59400.015.3049747
ACCGCGT249950.015.0169096
TCCGCAA77900.014.841372523
CGTTACG16400.014.8154921
AAGAACG258350.014.67195143
Produced by FastQC (version 0.11.5)