FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51989679
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT36050.012.48167611
ACCCGGC100100.012.2320525
ATAGCTC96900.011.8179539
GTTGTCG24400.011.4338426
TCGTGTA39000.011.42218414
AGCTTAG106450.011.03240145
TAGCGTA8650.010.92399137
CACCCGG117650.010.9050344
ACACGGA111100.010.85443516
TAGCTCT111750.010.5494840
GGCATAG112050.010.54132535
TCAGACG94350.010.2581682
CGGACAG119000.010.20967919
TGTAGGG46800.010.095674517
GTAGGGA48100.09.96322218
TCACCCG128950.09.9145063
CGGCCCG128500.09.89364058
GTCGTGT45450.09.85077213
ATAGGGG125400.09.81381638
AACTCTA98050.09.70594945
Produced by FastQC (version 0.11.5)