FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44971151
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA504590.11220304323542886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA6300.011.42585737
ATAGGGG113550.010.65779238
GGGAGGT174900.010.2491543
GGCATAG116900.010.02528635
ACCCGGC52050.09.9494895
GTTGTCG19350.09.64684226
AGCTTAG124000.09.55608145
AGGGATC135950.09.3413782
GGAGGTC184000.08.921064
GGTCACT176500.08.7640297
CATAGGG137300.08.73244937
ATCGGTT52450.08.5856536
ATAATCG49700.08.5638723
GTAACGA43100.08.562718
CACCGAA309850.08.55565545
GAGCTTA138450.08.52633944
GCATAGG144900.08.4141536
GTCACTC182800.08.4127358
TCACCCG67950.08.3185773
TAATCGG54100.08.24094
Produced by FastQC (version 0.11.5)