FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46949223
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG482930.10286219220284008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG168850.013.96951145
GGGAGGT280950.012.9404033
GGCATAG185950.012.51284535
ATAGGGG189250.012.49700438
GTCACTC288150.012.01579958
GGTCACT286100.011.92897
GAGCTTA201100.011.77406644
ACTCTCC297200.011.71802311
TGGCATA208750.011.27545534
GGAGGTC318700.011.2522624
CATAGGG221700.010.749042537
CACTCTC324700.010.67015410
GAGGTCA346800.010.6065045
AGGTCAC341300.010.5269196
TCACTCT340500.010.5119989
CGTGGCG128550.010.413441
GCATAGG231200.010.30721236
GTGGCGA130850.010.0926762
TGGCGAC134500.09.8855463
AGCATAG110550.09.79280945
Produced by FastQC (version 0.11.5)