FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44619432
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCCTCTCTCCTCCAGCCTCTCACACTCTCCTCAGCTCTCTCATCTCCT480980.10779608310567468No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG457690.10257638420856634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG162300.013.88247345
GGGAGGT238200.013.7328043
ATAGGGG167200.013.56793238
TCGCTCG36000.013.5597324
GGCATAG170050.013.25918335
CACACTC242050.012.4814424
GTTGTCG19750.012.41533726
TCCCTCT269450.012.3572023
TCACACT253400.012.14429423
GAGCTTA185800.012.13871844
TGGCATA190000.012.10327734
TTCCCTC233800.012.0857592
GGAGGTC271350.011.8905554
CCCTCTC268150.011.797514
GTCACTC262700.011.7603318
CTCACAC260950.011.6808222
GGTCACT277300.011.5630957
CTTCCCT271000.011.4560441
TCGCCTC65250.011.44897659
TCTCGCC69800.011.21845157
Produced by FastQC (version 0.11.5)