FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50417546
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG86000.012.76767938
GGCATAG93150.011.59429335
AGCTTAG98400.011.12639645
CATAGGG95500.011.07332937
GCATAGG102900.010.38637736
GAGCTTA119500.09.42633144
GTCACTC167050.09.2388848
TGGCATA118600.09.1637734
ACTCTCC182500.08.70456511
GGGAGGT200200.08.4471073
GGTCACT178100.08.4250217
GTTGTCG21400.08.307425526
GGCGTAG47250.08.1429235
CGTAGGG46150.08.14195137
CGTAACG13900.08.09077542
GGAGGTC208850.08.0658624
GCGTAGG47250.07.95244236
ACACTCG70000.07.8717534
TAGGCTA76050.07.661996410
TCACTCT221100.07.6417549
Produced by FastQC (version 0.11.5)