FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50022109
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG818490.16362564801096252No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT656260.13119398864210224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG26650.020.11527321
TTCGACG23300.019.7911576
GCCGGCT244350.018.8202881
CCGGCTT275850.018.461912
ACGCGTC32750.017.85883140
AGACCGC188450.017.7853874
GACCGCG192800.017.4074445
TTCACGC286800.017.184487
TCACGCT288700.017.0402228
ACCGCGT198150.017.005566
CAGACCG199300.016.963873
CGGCTTC322950.016.1176323
CACGCGT37750.016.08932339
CGGTATA36900.015.80632320
GTTCGAC30100.015.6189925
TCGACGA31050.015.57578857
CCGCGTT219250.015.4408117
CGCGTTC219900.015.3337948
GTTACGA36650.015.24139722
AAGCGCG38750.015.21240518
Produced by FastQC (version 0.11.5)