FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50022109
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG530120.1059771390286643No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA523800.10471369769715227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT263200.012.8731423
GGAGGTC275500.012.3150594
GTTGTCG32500.012.18196926
AGCTTAG221750.011.80547545
GGCATAG226000.011.45051935
GGTCACT286400.011.4152837
ATAGGGG229300.011.38594838
GTCACTC308000.010.8485158
GAGCTTA242350.010.67196544
TGGCATA249400.010.45668734
GAGGTCA334350.010.309165
TGGGAGG271400.010.2209312
ACTCTCC329300.010.18096811
AGGTCAC345300.010.1066596
TAATCGG58450.09.7000244
ATATAGC52050.09.6399886
GTAACGA56650.09.49041718
ATAATCG65350.09.3641593
CTGGGAG337900.09.3142071
ATCGGTT66200.09.31296
Produced by FastQC (version 0.11.5)