FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47542323
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2170900.4566247215139235No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA578730.12172943253109444No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG483220.1016399640379373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT399900.027.31460245
AGACCGC448950.024.7609374
CAGACCG454750.024.4748133
TTCGACG27050.024.2860096
GACCGCG469350.023.5116335
ACCGCGT485750.022.7363536
AGAACGA487550.022.45484244
CCGCGTT496850.022.200967
CGCGTTC500850.022.019168
CTCAGAC545150.021.9344621
GCGTTCT512000.021.605559
TCAGACC530600.021.4326212
AAGAACG537800.020.30739843
TACGGAG535700.020.2538634
AATACGG542350.020.19610832
ATACGGA541900.020.18459933
CGGTATA39300.019.81066520
CAATACG557750.019.74600831
ACGGAGA558450.019.47711435
CGTTCTC581500.019.37572310
Produced by FastQC (version 0.11.5)