FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52519491
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1479040.28161735230830776No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT908730.17302719099086472No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG607110.11559708375696177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG38100.024.838456
GCCGGCT357000.022.4085671
CGGTATA59800.021.4996420
CCGGCTT434300.020.8485012
TCGACGA45850.020.6396277
GTTCGAC44800.020.5922725
AGACCGC243550.019.7245984
CGTTACG37800.019.62272521
CAGACCG249450.019.3486423
TTCACGC465750.019.1697067
GACCGCG251200.018.8820615
TCACGCT479450.018.6349628
CGACGAG50350.018.4367898
CGGCTTC506250.018.312133
GTTCCTA105050.018.03733615
ACCGCGT265450.018.0115936
ACGCTCA540150.016.69054410
CCGCGTT292750.016.346997
TCGTTAC26300.016.34372144
CGCGTTC300950.015.9081598
Produced by FastQC (version 0.11.5)