FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52519491
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA944130.17976754572887996No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG747350.14229955122756235No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG649390.1236474283423653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT321850.014.83169653
GTTGTCG49750.014.82815626
GGAGGTC331200.014.3450024
GAGGTCA346750.013.6950615
AGGTCAC362700.013.2477916
GTCACTC346250.013.2096318
ATAGGGG280450.012.79995238
ATATAGC68350.012.7308956
GGCATAG276850.012.62466135
AGCTTAG287300.012.36798545
ACTCTCC376200.012.06831411
TGTCGTT63800.011.95660928
TGGGAGG318500.011.9167382
GGTCACT388350.011.793797
TGGCATA302600.011.66198834
TCACTCT399900.011.6005169
CACTCTC390900.011.551210
GAGCTTA306300.011.53415444
GCTAAGT65150.011.52846414
GTCGTTC68250.011.14373129
Produced by FastQC (version 0.11.5)