FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48645701
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT615090.12644282790785563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT139300.022.1315466
AGACCGC141050.022.0165234
GACCGCG139850.021.9318715
ACGTCCG31400.021.13712132
CCGCGTT147850.020.835997
CAGACCG151250.020.7103753
CGCGTTC151750.020.313878
CGTCCGC34750.018.96995433
GAACGAT186400.016.7433445
AACGCTA170450.016.52551323
GCGTTCT188150.016.4898829
TCAGACC196350.016.3768772
ACGCTAC173200.016.34126924
CGCTACC178850.015.82503525
TCACGCT186800.015.7916178
TCGTATG45150.015.20034945
TTCGACG16750.015.1811936
ACGCTCA202200.015.16543210
AAAACGC188600.015.1615621
GTTCCTA95000.015.06185615
Produced by FastQC (version 0.11.5)