FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48645701
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG487650.10024524058148529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT33700.018.23260111
TCGTGTA38950.016.06375314
GGTCGCC42450.015.684257543
GTTGTCG27350.013.99037626
AGCTTAG180200.013.567368545
GGCATAG181400.013.4808535
GTCGTGT47900.013.20368713
GGAGGTC271650.013.150484
ATAGGGG193600.012.92198938
GGGAGGT296100.012.7541493
GAGCTTA203900.012.05762544
TGGCATA205000.011.99480234
ACCCGGC85050.011.8507945
CGTCGTG53100.011.82594612
GTAGGGA56500.011.62725718
TCACCCG91750.011.4240233
TCTCGGT57100.011.34744136
TGGTCGC75700.011.29179942
TGTAGGG58700.011.23152117
GGTCACT313200.011.1205667
Produced by FastQC (version 0.11.5)