FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58121343
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG688270.1184194935068861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC101400.021.3053474
CAGACCG107150.020.4349963
GACCGCG111550.019.3062745
GTTCCTA144750.018.7140915
ACCGCGT117650.018.3587686
TTCGACG25150.017.623516
CCGCGTT128450.016.867727
CGCGTTC130450.016.5573718
CCTACTC165600.016.38505218
CCCGTAT43900.016.1064741
CCCGGAT114500.016.0676351
CTCGTAT76350.016.014971
GCCGGCT238600.015.5910361
GTCCGTT44200.015.3857981
AGTTACC177050.015.36353934
CCGGCTT283100.015.3239612
CCGTACG49050.015.2319144
CGTACGC50350.014.9727465
CTCCGAT53900.014.8729321
AACATGC205850.014.7583915
Produced by FastQC (version 0.11.5)