FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58121343
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG890830.15327071846911727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT389700.016.0083963
GGAGGTC399000.015.1889934
GAGGTCA446450.013.86545855
GGTCACT426600.013.8377857
AGCTTAG338800.013.725759545
GGCATAG340350.013.61925535
ATAGGGG355750.013.44279238
GTTGTCG29500.013.19219126
GTCACTC448950.013.1485388
TGGGAGG371400.013.1333242
ACTCTCC468500.012.58355411
GAGCTTA371100.012.5544444
TGGCATA374550.012.49578134
TCACTCT492800.012.1761089
CTGGGAG440550.012.1160841
CACTCTC498150.011.73026210
CATAGGG413250.011.34276537
AGGTCAC554100.011.11710556
GCATAGG428950.010.963536
TAGGGGA545950.010.81969239
Produced by FastQC (version 0.11.5)