FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47224476
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG144300.014.33122145
ATAGGGG146300.014.30260238
GGCATAG157650.013.1158435
GAGCTTA168100.012.34231844
GTCACTC237750.012.3116228
GGGAGGT259600.012.14870553
GGTCACT247100.012.0636997
GTTGTCG22450.011.72471326
CATAGGG183600.011.47042837
TGGCATA186100.011.183619534
ACTCTCC266100.011.17820911
GGAGGTC289350.010.8531774
GCATAGG195850.010.776113536
TCACTCT295550.010.247189
AGGTCAC303550.010.1833066
GAGGTCA319000.010.0427045
CACTCTC309000.09.67739810
TGGGAGG256900.09.0566262
TAGGGGA325400.08.95439139
GCTCTGT335600.08.84371622
Produced by FastQC (version 0.11.5)