FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48437224
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT541250.11174257220025656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG23750.022.26246
GAACGAT153800.021.41667645
TTCGGCG84600.019.46740344
ACCGCGT168200.019.3022566
TCGGCGC84850.019.17139645
GACCGCG171550.018.9134985
AGACCGC171950.018.908764
CGCGTTC175450.018.5431528
CCGCGTT176050.018.4671767
CAGACCG183400.017.8877433
CCGTACG92700.017.4763244
CGTACGC94300.017.2513855
TCGACGA30850.017.1388387
AGAACGA197050.016.75023744
CGGTATA39750.016.5279120
CGACGAG31350.016.506658
TCCGTAC106550.015.8804113
TACGCCA104450.015.7893477
CGTTACG15000.015.74970821
TCAGACC217800.015.5378482
Produced by FastQC (version 0.11.5)