FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48662006
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTC667760.13722410046145653No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG591880.12163082631653122No Hit
CTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTC542350.11145245430284974No Hit
CTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTG516320.10610331189388286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCGC114450.017.33074445
AGACCGC72900.016.2756084
CGTACGC124400.016.1254355
CCGTACG123600.016.10234
GCCGGCT212050.015.8103991
TTCACGC214400.015.6802377
TCACGCT214900.015.5914348
TCCGTAC129500.015.5425373
CCGGCTT239900.015.4481362
TTCGGCG131050.015.41065344
TTCGACG17400.015.38089756
GTTAGTT140500.015.1584744
TACGCCA132700.015.1157667
GTACGCC135450.014.9765416
CAGACCG80800.014.93508153
ACCGCGT81150.014.743726
CGCCCCG137100.014.54956523
GTCCGTT27200.014.3303041
CGGGGCG139850.014.30275433
CGTTACG15450.014.1184821
Produced by FastQC (version 0.11.5)