FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48662006
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG779350.16015574861422688No Hit
CTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCC534940.10992970573387377No Hit
GGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGC520770.10701778303179692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG250000.015.50525945
GGGAGGT349700.015.4721043
GGCATAG272000.014.16259935
GGAGGTC388300.013.8355654
ATAGGGG284000.013.72263838
GAGCTTA282650.013.70382144
TGGCATA286700.013.45115334
TGGGAGG301500.013.4461342
GGTCACT436000.012.0897067
CTGGGAG365250.012.0565971
ACTCTCC452400.011.91997611
CATAGGG333400.011.66910337
TCAGACG117550.011.5596392
GTCACTC466200.011.4609688
GCATAGG350100.011.13818736
GCTCTGT475850.011.10091722
CTCCCCA366950.010.88844914
TCTCCCC487250.010.84578813
AGGCTCT367250.010.7950920
GTTGTCG23200.010.76380526
Produced by FastQC (version 0.11.5)