FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47599007
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT664610.13962686238391486No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG501850.10543287174037054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG38000.024.9860176
GAACGAT238200.023.24545545
AGACCGC255550.021.7480954
GACCGCG257250.021.3678995
GTTCGAC43850.021.3460465
CAGACCG264900.021.1843243
ACCGCGT262250.020.9678526
CCGCGTT268750.020.4774867
TCGACGA46400.020.4627087
CGCGTTC272250.020.2390238
CGACGAG46100.020.205438
GCCGGCT177000.020.1746121
CGGTATA58800.019.89939320
CCGGCTT212650.019.0461522
TCAGACC310550.018.3673522
AATACGG302350.018.23157932
ATACGGG120900.017.80958733
TTCACGC221300.017.8021897
AGAACGA312900.017.76068144
CTCAGAC352650.017.5464941
Produced by FastQC (version 0.11.5)