FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47599007
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG31850.013.98330626
ATATAGC55000.010.3929596
ATAGGGG126650.010.339025538
TGTCGTT44050.010.21275128
GGCATAG129200.09.99412135
AGGGATC144450.09.9751762
AACTCTA108150.09.80054545
GGGAGGT191600.09.6236973
GTCGTTC44900.09.61834529
AGCTTAG137500.09.52524445
GGAGGTC200600.08.9560854
TCGTTCC51250.08.77781930
CAGGGAT186100.08.6986541
GCTAAGT55650.08.61325914
ACTCTCC211100.08.58145111
GTCACTC207800.08.5553438
TAATCGG48800.08.488764
TCAGACG64600.08.4689952
CGTGGCG79800.08.3795961
TATAGCT68500.08.3769757
Produced by FastQC (version 0.11.5)