FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47557297
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1832770.3853814484031757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT279600.031.72817445
AGAACGA327050.027.15244544
AGACCGC340700.026.2047564
CAGACCG354600.025.260283
GACCGCG354600.025.1465175
ACCGCGT358750.024.8366246
CCGCGTT367300.024.2456137
CGCGTTC370200.024.04338
TACGGAG374600.023.6760834
AATACGG376850.023.55902532
ATACGGA376850.023.5348733
TCAGACC393500.023.0205672
AAGAACG388750.022.87779643
CAATACG398900.022.35898231
ACGGAGA401350.022.16503535
CTCAGAC443000.021.6277261
GCGTTCT414450.021.6119659
CGGAGAG418600.021.26776536
CCCAATA427800.020.84622229
TTCGGCG77450.020.36350644
Produced by FastQC (version 0.11.5)