FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47557297
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG122150.014.78933145
GGCATAG127200.014.18560935
ACCCGGC91900.013.8827015
ATAGGGG135400.013.44258538
ATAGCTC89000.013.42324439
CATAGGG139300.013.08235537
TCACCCG103150.013.0025123
GAGCTTA145850.012.5097544
GCATAGG146650.012.39588936
TAGCTCT99550.012.06837740
TGGCATA151900.011.84924434
ACCTAGC52600.011.7624216
CGGACAG107700.011.44898919
GTCACTC217750.011.2926718
GGTCACT214050.011.2463697
CCGGACA119450.011.1691512
ACTCTCC222250.011.16547311
CACCCGG121850.010.7658324
GGGAGGT256150.010.7444053
TGATAGC116100.010.71630237
Produced by FastQC (version 0.11.5)