FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52547072
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG973820.18532336111896017No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA954790.18170184629887653No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG932750.17750751174109186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG35100.014.86852326
GGGAGGT456100.014.7683793
GGAGGTC475450.013.9832124
AGCTTAG332250.013.59189245
CACCCGG151150.013.0671951
GGTCACT507600.012.9308117
ACTCTCC520400.012.87219211
GGCATAG347800.012.86231835
ATAGGGG351300.012.81264838
GTCACTC520950.012.7419818
TGGGAGG381700.012.4420252
GAGCTTA367950.012.26091644
TGGCATA369750.012.13463434
CACTCTC556200.011.91016510
TGTCGTT43900.011.88867928
CTGGGAG443250.011.4545721
AGGGATC243050.011.3202452
AGCATAG181250.011.1907945
GCTCTGT600350.011.07148922
AGGCTCT423900.010.962604520
Produced by FastQC (version 0.11.5)