FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53052592
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2546300.47995769933352167No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG2207220.41604376276280713No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT1099190.2071887458392231No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG1063690.20049727259320335No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA816120.15383225762089062No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG815110.15364188049473623No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA726190.13688115370498768No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC579310.10919541876483621No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG559430.10544819374706518No Hit
CGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGT556800.10495245925024738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT509950.024.88926945
GCCGGCT483800.022.1496161
AGACCGC585750.021.914064
CAGACCG595250.021.5945533
CCGGCTT572650.021.1582852
TTCGACG17450.021.1422796
GACCGCG608450.021.0299425
AGAACGA606550.020.93270944
ACCGCGT622700.020.5703666
TCAGACC645600.020.084632
CCGCGTT638650.020.0531067
CGCGTTC646350.019.8281338
TCACGCT591750.019.8062788
TTCACGC594250.019.749457
GCGTTCT663350.019.3471189
GTTAGTT278750.019.20733544
CGGCTTC644050.019.2038613
CTCAGAC727350.018.7433831
AAGAACG691450.018.33970343
AATACGG710300.017.89469732
Produced by FastQC (version 0.11.5)