FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48658459
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG897230.18439342684485754No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT797480.16389339415783802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT232750.021.5378061
AGACCGC182400.021.4176224
ACCGCGT184600.021.0005786
GACCGCG184950.020.9641515
AACGCTA253600.020.6193423
ACGCTAC254500.020.51107824
CAGACCG192200.020.4309433
TTCGACG38150.020.0521986
CGCTACC261400.019.92666425
CCGCGTT194750.019.84837
CGCGTTC195050.019.8174868
CCGGCTT278050.019.7577362
TCACGCT272300.019.500058
TTCACGC276450.019.4924557
AAAACGC276050.018.9913621
GAAAACG281450.018.6428420
CGGCTTC312500.018.1034623
AAACGCT291600.018.02491422
ACGCTCA299700.017.8969810
TCAGACC230200.017.5978452
Produced by FastQC (version 0.11.5)