FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48658459
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG159950.011.71738638
GGCATAG158000.011.5913935
GTTGTCG24350.011.45582726
GGGAGGT243300.011.2259993
AGCTTAG161150.011.11281445
GGAGGTC238000.010.8420584
GGTCACT242850.010.0971927
TGGCATA182200.010.0515534
GAGCTTA186950.09.63947344
CATAGGG192850.09.47349837
ACTCTCC265700.09.2989911
GTCACTC266300.09.2341728
GAGGTCA287200.09.2112025
GCATAGG201350.09.10725736
CACTCTC279050.08.91038210
AGGGATC162800.08.6582622
TAATCGG43400.08.6123654
TCACTCT304500.08.5123329
TAGGGGA292950.08.509747539
ACCCGGC66200.08.2643975
Produced by FastQC (version 0.11.5)