FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40806084
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG94600.012.77148445
GGCATAG95600.012.708837535
ATAGGGG101850.012.08421938
CATAGGG112050.010.72319937
GAGCTTA114000.010.69673244
TGGCATA116150.010.63473934
GGGAGGT187150.010.5319863
GCATAGG120950.010.02712136
GGTCACT175300.09.99768457
GGAGGTC186550.09.92624
TCAGACG69450.09.9172572
ACCCGGC57900.09.8310695
GTCACTC180350.09.805068
GTCAGGA157050.09.5022051
TATCCTA76050.09.3486015
TAGGCTA70100.09.08235510
ATAGCTC57200.08.8899339
AGGGATC98400.08.829472
ATCCTAG90400.08.7354276
CGTAGGG59550.08.57707237
Produced by FastQC (version 0.11.5)