FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60505864
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG947620.1566162248340095No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT726090.12000324464418854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG26550.018.7264396
CGTTACG28600.018.56510521
GCCGGCT289950.017.6804521
TCACGCT300400.017.3889878
CCGGCTT327750.017.24542
TTCACGC312350.016.6877027
AACGCTA317800.015.92921723
ACGCTAC318550.015.87787724
GTCCGTT46550.015.6219861
CGCTACC325300.015.54151925
ACGCTCA344550.015.34361210
CGGCTTC389650.014.8984593
AAAACGC343600.014.85705821
TCGACGA35700.014.682497
GAAAACG346950.014.67996820
AAACGCT358950.014.240684522
GTTACGA40000.014.00541122
CACGCTC377100.013.9476159
CGGTATA48750.013.10567320
GTTCGAC39000.013.0965715
Produced by FastQC (version 0.11.5)