FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60505864
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG768030.1269348042034405No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA618700.10225455172411058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG31300.014.16097426
AGCTTAG300000.012.86649245
GGGAGGT445550.012.719573
GGAGGTC471300.011.8430894
GGCATAG338250.011.27629235
GAGGTCA506500.011.2287895
ATAGGGG346250.011.14587438
GAGCTTA352700.010.93116244
GGTCACT502550.010.7256117
GTCACTC509250.010.6595968
TGGCATA362050.010.58452834
ACTCTCC515800.010.55463311
AGGTCAC541000.010.3458846
TGGGAGG408350.09.9500652
CACTCTC542000.09.90748210
ATATAGC55550.09.881446
TCACTCT569500.09.6937329
CGTGGCG178550.09.4881041
TAGGCTA114050.09.44822910
CTGGGAG484400.09.2332911
Produced by FastQC (version 0.11.5)