FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51555657
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA694350.1346796918910373No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG567090.10999568873693144No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG558850.10839741601973961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG33100.015.2928426
GGGAGGT287200.014.3664613
AGCTTAG215100.013.09548445
GGAGGTC314700.012.8894344
ATAGGGG222550.012.730391538
GGCATAG220800.012.65713435
GGTCACT314700.012.4186687
GTCACTC316100.012.3780118
GAGGTCA344450.011.9072935
GCGTCGT25450.011.75894411
ACTCTCC335650.011.73156311
GAGCTTA242950.011.63256544
TGGCATA250300.011.30014234
AGGTCAC360200.011.2746916
CACTCTC352150.011.18187910
TCACTCT366750.011.0925859
ATATAGC55600.010.9259736
TGGGAGG285650.010.6241982
TGTCGTT50500.010.38059328
AGGGATC170550.010.1963852
Produced by FastQC (version 0.11.5)