FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51637392
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT866700.16784348830010626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT188150.022.27324145
AGACCGC195300.021.5767084
TTCGACG18200.020.6388856
CAGACCG208550.020.2705823
GACCGCG230900.018.2110525
AGAACGA232600.018.06567244
ATACGGA238850.017.57736233
TACGGAG238050.017.52408634
AATACGG243600.017.3170932
ACCGCGT244700.017.2165036
GCGTTCT246500.017.1457319
CCGCGTT248350.016.9725287
CGCGTTC252950.016.6818548
TTCGGCG89900.016.1644144
CTCAGAC295850.016.129421
ACGGAGA263300.015.92930735
TCAGACC270750.015.9129232
CAATACG267550.015.86779531
AACGCTA152200.015.5914523
ACGCTAC153050.015.49016224
Produced by FastQC (version 0.11.5)