FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51637392
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG157350.014.71293745
GGCATAG166600.013.74740235
ATAGGGG176250.013.14792438
ACCCGGC76100.012.8355725
GAGCTTA182300.012.68690144
GGGAGGT283450.012.5059183
TGGCATA197250.011.7595434
CATAGGG197900.011.68678837
GCATAGG206650.011.25726736
GGAGGTC297350.011.1038284
TCAGACG84400.011.0397722
GGTCACT291650.010.965857
ATAGCTC82850.010.91648139
ACTCTCC299250.010.90916711
TCACCCG96700.010.7994823
GTCACTC299550.010.7967398
GTTGTCG23900.010.26086526
TAGCGTA7400.010.03298337
GTTCTAT194750.010.0071361
CACTCTC331000.09.97150910
Produced by FastQC (version 0.11.5)